Line-scanning fiber bundle endomicroscopy with a virtual detector slit

Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle.

We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an opti...

Fiber-optic nonlinear endomicroscopy with focus scanning by using shape memory alloy actuation.

A miniature fiber optic endomicroscope with built-in dynamic focus scanning capability is developed for the first time for 3-D two-photon fluorescence (TPF) imaging of biological samples. Fast 2-D lateral beam scanning is realized by resonantly vibrating a double-clad fiber cantilever with a tubular piezoactuator. Slow axial scanning is achieved by moving the distal end of the imaging probe wit...

A scanning slit optical microscope.

Toward endoscopes with no distal optics: video-rate scanning microscopy through a fiber bundle.

We report a step toward scanning endomicroscopy without distal optics. The focusing of the beam at the distal end of a fiber bundle is achieved by imposing a parabolic phase profile across the exit face with the aid of a spatial light modulator. We achieve video-rate images by galvanometric scanning of the phase tilt at the proximal end. The approach is made possible by the bundle, designed to ...

3D Slit Scanning with Planar Constraints*

We present a planarity constraint and a novel three-dimensional (3D) point reconstruction algorithm for a multiview laser range slit scanner. The constraint is based on the fact that all observed points on a projected laser line lie on the same plane of laser light in 3D. The parameters of the plane of laser light linearly parametrize a homography between a pair of images of the laser points. T...